Clathrin heavy-chain 1,2 antibody AS10 690 from Agrisera

Product Specs
ItemClathrin heavy-chain 1,2 antibody
CompanyAgrisera
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAS10 690
ApplicationsImmunolocalization, Immunofluorescence, Western blot
FormatLyophilized
ReactivityActinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacumAS07 260
HostRabbit
ClonalityPolyclonal
ImmunogenKLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
NCBI Gene AliasesAtCHC1, clathrin heavy chain 1, CHC1, F11B9.30
Research AreaCell Biology
PurityImmunogen affinity purified serum in PBS pH 7.4
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
Molecular Weight193 : 170 kDa (Arabidopsis thaliana)

Specifications/Features

1: 2000 with standard ECL (WB), 1: 2400 (IL), 1:400 (IF)

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Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) Cargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plantsNature CommunicationsDecember 1, 2021Yutaro Shimizu et al.immunofluorescence staining with the anti-CHC antibody that recognizes both CHC isoforms of A. thaliana (Agrisera, AS10 690). The immunostaining images showed that endogenous CHCs colocalized well with CLC2-GFP and AP1M2-GFP

(2) Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in ArabidopsisProceedings of the National Academy of SciencesOctober 6, 2020Masaru Fujimoto, Kazuo Ebine, Kohji Nishimura, Nobuhiro Tsutsumi, Takashi Uedaand CHC was prepared as described previously (8). The anti-CHC antibody was purchased from Agrisera (AS10 690). Antibodies were diluted for immunoblotting as follows: anti-GFP, ×1,000; anti-His-tag, ×1,000; anti-CHC,

(3) Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the CiliumCurrent BiologySeptember 1, 2019Peeyush Ranjan, Mayanka Awasthi, William J. Snell

(4) Disruption of endocytosis through chemical inhibition of clathrin heavy chain functionNature Chemical BiologyJune 1, 2019Wim Dejonghe et al.detect the proteins. All antibodies were diluted in TBS-T with 5% (w/v) skimmed milk. Anti-CHC (1/3.000, AS10 690 Agrisera) anti-AtpB (1/4.000, AS05085 Agrisera) were detected with horseradish peroxidase (HRP)-linked

(5) Clathrin in Chara australis: Molecular Analysis and Involvement in Charasome Degradation and Constitutive EndocytosisFrontiers in Plant ScienceJanuary 26, 2017Marion C. Hoepflinger, Margit Hoeftberger, Aniela Sommer, Christina Hametner, Ilse Foissnerand 50 mM glycine in PBS (blocking buffer), 2 h incubation with primary antibody anti-CHC (Agrisera AS10690; Vännäs, Sweden) at a concentration of 0.01 mg.ml−1; 3 × 30 min wash with PBS, overnight incubation

(6) Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomainsNature communicationsSeptember 29, 2016Wattelet-Boyer V, Brocard L, Jonsson K, Esnay N, Joubès J, Domergue F, Mongrand S, Raikhel N, Bhalerao RP, Moreau P, et al.and then washed four times with MTSB. Antibody dilutions were as follows: rabbit anti-CHC (Agrisera, AS10 690) 1/300, rabbit anti-PIN2 (ref. 66) 1/1,000; rabbit anti-MEMB11 (ref. 29) 1/300, TRITC-coupled donkey

(7) Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in ArabidopsisThe Plant cellOctober 1, 2015Derbyshire P, Ménard D, Green P, Saalbach G, Buschmann H, Lloyd CW, Pesquet E.

(8) Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental rolesJournal of experimental botanyAugust 1, 2015Peter Grones, Xu Chen, Sibu Simon, Walter A Kaufmann, Riet De Rycke, Tomasz Nodzynski, Eva Zažímalová, Jirí Friml

(9) Identification of novel candidate phosphatidic acid-binding proteins involved in the salt-stress response of Arabidopsis thaliana rootsThe Biochemical journalMarch 15, 2013Fionn McLoughlin, Steven A Arisz, Henk L Dekker, Gertjan Kramer, Chris G de Koster, Michel A Haring, Teun Munnik, Christa Testerink

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Anti-Clathrin heavy-chain 1,2

Clathrin heavy-chain 1,2 antibody from Agrisera

Description

Specifications/Features

Agrisera

Citations (9)

Reviews

Clathrin heavy-chain 1,2 antibody from Agrisera

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